Immunogenic and protective properties of recombinant proteins from a highly pathogenic Campylobacter jejuni clone associated with sheep abortion
نویسندگان
چکیده
....................................................................................................................................... iii CHAPTER 1. GENERAL INTRODUCTION ................................................................................. 1 THESIS ORGANIZATION .......................................................................................................................... 3 LITERATURE REVIEW ......................................................................................................................... 4 Campylobacter: microbiology and genetics ................................................................................................. 4 Human campylobacteriosis ................................................................................................................................. 6 Ovine campylobacteriosis .................................................................................................................................... 8 Virulence factors of C. jejuni ............................................................................................................................. 12 Treatment ................................................................................................................................................................ 21 Prevention and Vaccines ................................................................................................................................... 22 REFERENCES ............................................................................................................................................ 24 CHAPTER 2. IMMUNOGENIC AND PROTECTIVE PROPERTIES OF RECOMBINANT PROTEINS FROM A HIGHLY PATHOGENIC CAMPYLOBACTER JEJUNI CLONE ASSOCIATED WITH SHEEP ABORTION ................................................................................. 43 INTRODUCTION ................................................................................................................................................. 44 MATERIALS AND METHODS ........................................................................................................................... 49 RESULTS ................................................................................................................................................................... 59 DISCUSSION ............................................................................................................................................................ 64 CONCLUSION .......................................................................................................................................................... 69 ACKNOWLEDGEMENTS ..................................................................................................................................... 70 REFERENCES .......................................................................................................................................................... 70 CHAPTER 3. GENERAL CONCLUSION ..................................................................................... 86 ACKNOWLEDGMENTS ................................................................................................................ 88
منابع مشابه
Genetic diversity and antimicrobial susceptibility of Campylobacter jejuni isolates associated with sheep abortion in the United States and Great Britain.
Campylobacter infection is a leading cause of ovine abortion worldwide. Historically, genetically diverse Campylobacter fetus and Campylobacter jejuni strains have been implicated in such infections, but since 2003 a highly pathogenic, tetracycline-resistant C. jejuni clone (named SA) has become the predominant cause of sheep abortions in the United States. Whether clone SA was present in earli...
متن کاملEmergence of a tetracycline-resistant Campylobacter jejuni clone associated with outbreaks of ovine abortion in the United States.
Campylobacter infection is one of the major causes of ovine abortions worldwide. Historically, Campylobacter fetus subsp. fetus was the major cause of Campylobacter-associated abortion in sheep; however, Campylobacter jejuni is increasingly associated with sheep abortions. We examined the species distribution, genotypes, and antimicrobial susceptibilities of abortion-associated Campylobacter is...
متن کاملInsights into potential pathogenesis mechanisms associated with Campylobacter jejuni-induced abortion in ewes
BACKGROUND Campylobacter jejuni is commonly found in the gastrointestinal tract of many food-animals including sheep without causing visible clinical symptoms of disease. However, C. jejuni has been implicated in ovine abortion cases worldwide. Specifically, in the USA, the C. jejuni sheep abortion (SA) clone has been increasingly associated with sheep abortion. In vivo studies in sheep (the na...
متن کاملA comparative analysis of methylome profiles of Campylobacter jejuni sheep abortion isolate and gastroenteric strains using PacBio data
Campylobacter jejuni is a leading cause of human gastrointestinal disease and small ruminant abortions in the United States. The recent emergence of a highly virulent, tetracycline-resistant C. jejuni subsp. jejuni sheep abortion clone (clone SA) in the United States, and that strain's association with human disease, has resulted in a heightened awareness of the zoonotic potential of this organ...
متن کاملPathogenicity of an emergent, ovine abortifacient Campylobacter jejuni clone orally inoculated into pregnant guinea pigs.
OBJECTIVE To compare pathogenicity of an emergent abortifacient Campylobacter jejuni (IA 3902) with that of reference strains after oral inoculation in pregnant guinea pigs. ANIMALS 58 pregnant guinea pigs. PROCEDURES 12 animals were challenged IP with C jejuni IA 3902 along with 5 sham-inoculated control animals to confirm abortifacient potential. Once pathogenicity was confirmed, challeng...
متن کامل